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KMID : 0352819920080010001
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Kosin Medical Journal
1992 Volume.8 No. 1 p.1 ~ p.22
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Immunohistochemical Investigation for distributon of Type IV Collagen and febronectin in Hepatocellular Carcinoma
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Abstract
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It is well known that some of the basement membrane glycoproteins are sysnthesized and expressed in neoplastic hepatic tissue in spite of absence of an intact, organized basement membrane in the normal liver parenchyma
In an attempt to examine the distribution and expression patterns of basement meimbrane glycoproteins in hepatocellular carcinoma (HCC), immunohistochemical localization of type IV collagen and fibronectin is performed. Fifty cases of
HCC
are histologically analyzed and paraffen-embedded sections are histologically analyzed and paraffin-embedded sections are immunostained with mono clonal antibodies to type IV collagen and
fibronectin(Avidin-Biotin-Peroxidase Complex).
Positive reactions to type IV collagen and fibronectin are observed in extracellular spaces. Intracellular expression of fibronectin in some cases is observed.
The distribution patterns of these glycoproteins are categorized into four locations : perisinusoidal(Pattern I ), periacinar(Pattern II), stromal(Pattern III), and pericellular (Pattern IV) areas. Patterns I and II are
distributed
throughout the lesion, while patterns III and IV are observed in focal areas of the neoplastic tissues. These findings suggest that deposition of basement membrane glycoproteins in the extracellular matrix may be related to the histologic
types
of
HCC.
In well-differentiated neoplastic cells(Edmondson grades I and II) organized in trabecular and/or acinar pattern, type IV collagen and fibronectin are always detected in the sinusoidal surface as pattern I and/or II. In normal liver
prenchyma,
typeII collagen and fibronectin are also expressed in the sinusoidal surface. However, in less-differentiated neoplasms(Edmondson grades III and IV). sinusoids are almost absent and only fibrotic stroma with vascularized connective tissue is
reactive
to type IV collagen and fibronectin. These findings suggest that the expression of basement membrane glycoproteins may be influnced by the degree of differentiation.
Intracellular expression of fibronectin is noted in 30 cases of hepatocellular carcinoma(60%), while it is not observed in normal liver cells. The expression modes can be classified into 3 patterns 1)scattered, 2)stromal interface, or
3)mixed
pattern. The stromal interface pattern is frequently observed in highly differentiated tumors and clear cell type of HCC. significances of these findings are remained to be resolved.
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